The BBC3 Polyclonal Antibody (PAC021344) is a valuable tool for researchers investigating the role of BBC3, a key player in apoptosis and cell death regulation. This antibody, generated in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications.BBC3, also known as PUMA (p53 upregulated modulator of apoptosis), is a critical regulator of the intrinsic pathway of apoptosis, promoting cell death in response to various stress signals. Its involvement in apoptosis makes it a target of interest in cancer research, as dysregulation of apoptosis pathways is a hallmark of cancer development and progression.
By targeting BBC3 protein, researchers can study its expression levels and activity in different cell types, providing insights into its role in cell death mechanisms and potential implications for cancer therapy. This antibody is a valuable tool for studies aiming to understand the molecular mechanisms underlying apoptotic signaling pathways and their relevance in cancer biology.
Antibody Name:
BBC3 Antibody (PACO21344)
Antibody SKU:
PACO21344
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:1000
Species Reactivity:
Human
Immunogen:
Form:
Liquid
Storage Buffer:
Purified Rabbit polyclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Purification Method:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from 293 and MCF-7 cells using Puma Antibody.
Synonyms:
BBC3, JFY1, JFY-1,
UniProt Protein Function:
PUMA: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Interacts with MCL1 and BCL2A1. Interacts with BCL2 and BCL2L1/BCL-XL. By DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. Ubiquitously expressed. Belongs to the Bcl-2 family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:
Protein type:Mitochondrial
Chromosomal Location of Human Ortholog: 19q13.3-q13.4
Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of protein homooligomerization; apoptosis; reduction of endoplasmic reticulum calcium ion concentration; positive regulation of neuron apoptosis; release of sequestered calcium ion into cytosol; determination of adult life span; response to DNA damage stimulus; negative regulation of growth
NCBI Summary:
This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]
