The BBC3 Polyclonal Antibody (PAC017630) is a valuable tool for researchers studying the BBC3 protein, also known as PUMA (p53 upregulated modulator of apoptosis). This antibody, developed and validated for use in a variety of research applications, is raised in rabbits and exhibits high reactivity with human samples.BBC3/PUMA is a key regulator of apoptosis, playing a critical role in programmed cell death and the maintenance of tissue homeostasis. Its dysregulation has been linked to various diseases, including cancer, making it an important target for investigation in cancer biology and therapeutic development.The BBC3 Polyclonal Antibody specifically binds to the BBC3/PUMA protein, allowing for the detection and analysis of its expression in different cell types.
Researchers in the fields of oncology, cell biology, and molecular biology can rely on this antibody for their studies on apoptosis, cancer progression, and potential therapeutic interventions targeting BBC3/PUMA.By utilizing the BBC3 Polyclonal Antibody, researchers can gain valuable insights into the molecular mechanisms underlying apoptosis regulation and its implications in disease pathogenesis. This antibody serves as a reliable tool for advancing our understanding of BBC3/PUMA function and its potential as a therapeutic target in cancer treatment strategies.
Antibody Name:
BBC3 Antibody (PACO17630)
Antibody SKU:
PACO17630
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:1000-1:2000, WB:1:200-1:1000
Species Reactivity:
Human
Immunogen:
Synthetic peptide of human BBC3
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane: A549 cells, Primary antibody: PACO17630(BBC3 Antibody) at dilution 1/600, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes.
Background:
This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants.
Synonyms:
BCL2 binding component 3
UniProt Protein Function:
PUMA: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Interacts with MCL1 and BCL2A1. Interacts with BCL2 and BCL2L1/BCL-XL. By DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. Ubiquitously expressed. Belongs to the Bcl-2 family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:
Protein type:Mitochondrial
Chromosomal Location of Human Ortholog: 19q13.3-q13.4
Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of protein homooligomerization; apoptosis; reduction of endoplasmic reticulum calcium ion concentration; positive regulation of neuron apoptosis; release of sequestered calcium ion into cytosol; determination of adult life span; response to DNA damage stimulus; negative regulation of growth
NCBI Summary:
This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]
