The BBC3 Polyclonal Antibody (PAC017629) is a valuable tool for researchers studying BBC3, a gene involved in apoptosis and cell death regulation. This antibody, generated in rabbits, exhibits high specificity and sensitivity in detecting BBC3 protein levels in human samples. Validated for use in Western blot applications, this antibody enables precise analysis of BBC3 expression in various cell types, making it ideal for studies in oncology and cell biology.BBC3, also known as PUMA (p53-upregulated modulator of apoptosis), plays a crucial role in promoting apoptosis in response to cellular stress and DNA damage.
Dysregulation of BBC3 expression is implicated in cancer development and progression, making it a promising target for therapeutic intervention. By elucidating the mechanisms underlying BBC3-mediated apoptosis, researchers can gain insights into potential treatments for cancer and other diseases characterized by abnormal cell death processes.
Antibody Name:
BBC3 Antibody (PACO17629)
Antibody SKU:
PACO17629
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:1000-1:2000, WB:1:200-1:1000
Species Reactivity:
Human
Immunogen:
Synthetic peptide of human BBC3
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Gel: 10%SDS-PAGE,Lysate: 40 μg,,Primary antibody: PACO17629(BBC3 Antibody) at dilution 1/300 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 5 minutes.
Background:
This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants.
Synonyms:
Bcl-2-binding component 3
UniProt Protein Function:
PUMA: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Interacts with MCL1 and BCL2A1. Interacts with BCL2 and BCL2L1/BCL-XL. By DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. Ubiquitously expressed. Belongs to the Bcl-2 family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:
Protein type:Mitochondrial
Chromosomal Location of Human Ortholog: 19q13.3-q13.4
Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of protein homooligomerization; apoptosis; reduction of endoplasmic reticulum calcium ion concentration; positive regulation of neuron apoptosis; release of sequestered calcium ion into cytosol; determination of adult life span; response to DNA damage stimulus; negative regulation of growth
NCBI Summary:
This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]
