Based on the information provided in the URL for the BAZ2B Antibody (PAC057536), we can create a product description as follows:The BAZ2B Monoclonal Antibody (PAC057536) is a powerful tool for researchers studying BAZ2B, a key protein involved in chromatin remodeling and gene regulation. This antibody is specifically designed to target BAZ2B in human samples, providing reliable and accurate results for various experimental applications. With its high specificity and sensitivity, this antibody is suitable for use in techniques such as Western blotting and immunofluorescence, enabling researchers to explore the function and expression of BAZ2B in different cellular contexts.
BAZ2B is an essential component of chromatin remodeling complexes, playing a crucial role in transcriptional regulation and epigenetic processes. Dysregulation of BAZ2B has been linked to various diseases, including cancer and neurodevelopmental disorders, making it a promising therapeutic target for drug discovery and personalized medicine. By investigating the function of BAZ2B using this monoclonal antibody, researchers can gain valuable insights into the mechanisms underlying disease pathogenesis and potential treatment strategies.
Immunofluorescence staining of Hela cells with PACO57536 at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO57536 diluted at 1:800 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of PACO57536 diluted at 1:800 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
May play a role in transcriptional regulation interacting with ISWI.
Synonyms:
Bromodomain adjacent to zinc finger domain protein 2B (hWALp4), BAZ2B, KIAA1476
UniProt Protein Function:
BAZ2B: May play a role in transcriptional regulation interacting with ISWI. Belongs to the WAL family. 5 isoforms of the human protein are produced by alternative splicing.Protein type: Transcription regulationChromosomal Location of Human Ortholog: 2q24.2Cellular Component: nucleusMolecular Function: protein binding; DNA binding; zinc ion bindingBiological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent
UniProt Protein Details:
NCBI Summary:
This gene belongs to the bromodomain gene family. Members of this gene family encode proteins that are integral components of chromatin remodeling complexes. The encoded protein showed strong preference for the activating H3K14Ac mark in a histone peptide screen, suggesting a potential role in transcriptional activation. This gene may be associated with susceptibility to sudden cardiac death (SCD). [provided by RefSeq, Aug 2016]
