The BAZ1B Polyclonal Antibody (PAC020928) is a valuable tool for researchers studying BAZ1B, a protein involved in chromatin remodeling and gene regulation. This antibody is raised in rabbits and has high reactivity with human samples, making it ideal for use in Western blot applications. By binding specifically to the BAZ1B protein, this antibody allows for the detection and analysis of BAZ1B in various cell types, providing important insights into its function in processes such as transcriptional regulation and DNA repair.BAZ1B, also known as WSTF (Williams-Beuren syndrome transcription factor), is a key player in chromatin remodeling complexes that regulate gene expression and DNA accessibility.
Its role in controlling cellular processes such as DNA replication and transcription makes it an important target for research in developmental biology, cancer, and other diseases. Understanding the function of BAZ1B can provide valuable insights into the mechanisms underlying these conditions and pave the way for the development of targeted therapies.
Antibody Name:
BAZ1B Antibody (PACO20928)
Antibody SKU:
PACO20928
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:1000-1:2000, IHC:1:10-1:50
Species Reactivity:
Human, Mouse
Immunogen:
Synthetic peptide of human BAZ1B
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using PACO20928(BAZ1B Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
This gene encodes a member of the bromodomain protein family. The bromodomain is a structural motif characteristic of proteins involved in chromatin-dependent regulation of transcription. This gene is deleted in Williams-Beuren syndrome, a developmental disorder caused by deletion of multiple genes at 7q11.23.
Synonyms:
bromodomain adjacent to zinc finger domain, 1B
UniProt Protein Function:
WSTF: plays a central role in chromatin remodeling and acts as a transcription regulator. Apparently possesses tyrosine-protein kinase activity, but bears no sequence resemblance classical tyrosine kinase proteins. Involved in DNA damage response by phosphorylating Y142 of histone H2AX. H2AXpY142 plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at Y142, and is involved in the maintenance of chromatin structures during DNA replication processes. In the complex, it mediates the recruitment of the WICH complex to replication foci during DNA replication. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. In the WINAC complex, plays an essential role by targeting the complex to acetylated histones, an essential step for VDR-promoter association. Accumulates in pericentromeric heterochromatin during replication. Targeted to replication foci throughout S phase via its association with PCNA. Ubiquitously expressed with high levels of expression in heart, brain, placenta, skeletal muscle and ovary. Two alternatively-spliced human isoforms have been reported.
UniProt Protein Details:
Protein type:Protein kinase, Ser/Thr (non-receptor); Nuclear receptor co-regulator; Kinase, protein; EC 2.7.10.2; DNA replication; ATYPICAL group; BAZ family
Biological Process: chromatin-mediated maintenance of transcription; double-strand break repair; heart morphogenesis; histone phosphorylation; positive regulation of gene expression, epigenetic; regulation of transcription, DNA-dependent; response to DNA damage stimulus
NCBI Summary:
This gene encodes a member of the bromodomain protein family. The bromodomain is a structural motif characteristic of proteins involved in chromatin-dependent regulation of transcription. This gene is deleted in Williams-Beuren syndrome, a developmental disorder caused by deletion of multiple genes at 7q11.23. [provided by RefSeq, Jul 2008]
Bromodomain adjacent to zinc finger domain protein 1B; Williams syndrome transcription factor; Williams-Beuren syndrome chromosomal region 10 protein; Williams-Beuren syndrome chromosomal region 9 protein; hWALp2
