Anti-Bax Antibody (CAB15646)
- SKU:
- CAB15646
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
Anti-Bax Antibody (CAB15646)
The Bax Polyclonal Antibody (CAB15646) is a valuable tool for researchers studying the Bax protein, a key player in regulating programmed cell death, or apoptosis. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting Bax in human samples, making it suitable for use in Western blot applications. By binding to the Bax protein, this antibody enables precise detection and analysis of Bax expression in various cell types.The Bax protein is a critical component of the intrinsic apoptotic pathway, playing a pivotal role in cellular homeostasis and response to stress signals. Dysregulation of Bax expression has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases.
Therefore, studying Bax expression and activity is essential for unraveling the mechanisms underlying these conditions and developing potential therapeutic interventions.Overall, the Bax Polyclonal Antibody (CAB15646) offers researchers a reliable tool for investigating the role of Bax in cell death regulation and its implications in health and disease. Its high reactivity and specificity make it an ideal choice for studies in apoptosis, cancer biology, and other fields of research where Bax protein function is of interest.
| Antibody Name: | Anti-Bax Antibody |
| Antibody SKU: | CAB15646 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide of human Bax |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | PC-12, SH-SY5Y, mouse testis, rat testis |
| Immunogen: | A synthetic peptide of human Bax |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 581 |
| Uniprot: | Q07812 |
| Cellular Location: | Cytoplasm, Cytoplasm, Mitochondrion membrane, Single-pass membrane protein |
| Calculated MW: | 4kDa/12kDa/15kDa/18kDa/19kDa/21kDa/24kDa |
| Observed MW: | 21kDa |
| Synonyms: | BCL2L4, BAX |
| Background: | The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. |
| UniProt Protein Function: | Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. |
| NCBI Summary: | The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q07812 |
| NCBI GenInfo Identifier: | 728945 |
| NCBI Gene ID: | 581 |
| NCBI Accession: | Q07812.1 |
| UniProt Secondary Accession: | Q07812,P55269, Q07814, Q07815, Q8WZ49, Q9NR76, Q9NYG7 Q9UCZ6, Q9UCZ7, Q9UQD6, A8K4W1, |
| UniProt Related Accession: | Q07812 |
| Molecular Weight: | 19,718 Da |
| NCBI Full Name: | Apoptosis regulator BAX |
| NCBI Synonym Full Names: | BCL2 associated X, apoptosis regulator |
| NCBI Official Symbol: | BAXÂ Â |
| NCBI Official Synonym Symbols: | BCL2L4Â Â |
| NCBI Protein Information: | apoptosis regulator BAX |
| UniProt Protein Name: | Apoptosis regulator BAX |
| UniProt Synonym Protein Names: | Bcl-2-like protein 4; Bcl2-L-4 |
| Protein Family: | Protein |
| UniProt Gene Name: | BAXÂ Â |
| UniProt Entry Name: | BAX_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Bax antibody (CAB15646) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded Mouse kidney using Bax Rabbit pAb (CAB15646) at dilution of 1:50 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Rat kidney using Bax Rabbit pAb (CAB15646) at dilution of 1:50 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Human colon using Bax Rabbit pAb (CAB15646) at dilution of 1:50 (40x lens). |
 | Immunofluorescence analysis of L929 cells using Bax Rabbit pAb (CAB15646) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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