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Anti-Bax Antibody (CAB15633)[KO Validated]

SKU:
CAB15633
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
  • KO Validated Antibodies 1 Anti-Bax Antibody CAB15633KO Validated
  • KO Validated Antibodies 1 Anti-Bax Antibody CAB15633KO Validated
  • KO Validated Antibodies 1 Anti-Bax Antibody CAB15633KO Validated
  • KO Validated Antibodies 1 Anti-Bax Antibody CAB15633KO Validated
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Description

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Anti-Bax Antibody (CAB15633)[KO Validated]

The Bax Polyclonal Antibody (CAB15633) is a valuable tool for researchers studying apoptosis and cell death pathways. Bax is a key player in the regulation of programmed cell death, and this antibody, generated in rabbits, specifically targets the Bax protein in human samples.Validated for use in Western blot applications, this antibody enables precise detection and analysis of Bax expression levels in various cell types. Its high reactivity and specificity make it an ideal choice for studies in cancer research, as dysregulation of apoptosis pathways is a hallmark of many types of cancer.

By targeting Bax, researchers can gain insights into the mechanisms of cell death and potential therapeutic targets for diseases characterized by abnormal cell survival. This antibody is a valuable tool for understanding the role of Bax in apoptosis and its potential implications for cancer therapy.

Antibody Name:Anti-Bax Antibody [KO Validated]
Antibody SKU:CAB15633
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide of human Bax
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Raji, HeLa, HepG2, 293T, Mouse testis
Immunogen:A synthetic peptide of human Bax
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:581
Uniprot:Q07812
Cellular Location:Cytoplasm, Cytoplasm, Mitochondrion membrane, Single-pass membrane protein
Calculated MW:4kDa/12kDa/15kDa/18kDa/19kDa/21kDa/24kDa
Observed MW:21kDa
Synonyms:BCL2L4, BAX
Background:The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
UniProt Protein Function:Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
NCBI Summary:The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q07812
NCBI GenInfo Identifier:728945
NCBI Gene ID:581
NCBI Accession:Q07812.1
UniProt Secondary Accession:Q07812,P55269, Q07814, Q07815, Q8WZ49, Q9NR76, Q9NYG7 Q9UCZ6, Q9UCZ7, Q9UQD6, A8K4W1,
UniProt Related Accession:Q07812
Molecular Weight:19,718 Da
NCBI Full Name:Apoptosis regulator BAX
NCBI Synonym Full Names:BCL2 associated X, apoptosis regulator
NCBI Official Symbol:BAX  
NCBI Official Synonym Symbols:BCL2L4  
NCBI Protein Information:apoptosis regulator BAX
UniProt Protein Name:Apoptosis regulator BAX
UniProt Synonym Protein Names:Bcl-2-like protein 4; Bcl2-L-4
Protein Family:Protein
UniProt Gene Name:BAX  
UniProt Entry Name:BAX_HUMAN
Anti-Bax Antibody [KO Validated] (CAB15633)Western blot analysis of extracts of various cell lines, using Bax antibody (CAB15633) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-Bax Antibody [KO Validated] (CAB15633)Western blot analysis of extracts from normal (control) and Bax knockout (KO) 293T cells, using Bax antibody (CAB15633) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-Bax Antibody [KO Validated] (CAB15633)Immunofluorescence analysis of C6 cells using Bax antibody (CAB15633) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-Bax Antibody [KO Validated] (CAB15633)Immunofluorescence analysis of NIH/3T3 cells using Bax antibody (CAB15633) at dilution of 1:100. Blue: DAPI for nuclear staining.

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