The Bax Polyclonal Antibody (CAB12009) is a valuable tool for researchers investigating the role of the Bax protein in apoptosis, or programmed cell death. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the Bax protein, this antibody enables detection and analysis in a variety of cell types, making it ideal for studies in molecular biology and cancer research.The Bax protein is a key regulator of apoptosis, promoting cell death under certain conditions.
Dysregulation of Bax has been implicated in various diseases, including cancer, neurodegenerative disorders, and autoimmune diseases. Understanding the function and regulation of Bax is essential for developing targeted therapies that can either inhibit or promote apoptosis in disease contexts.Overall, the Bax Polyclonal Antibody (CAB12009) offers researchers a reliable tool for studying the Bax protein and its role in apoptosis, providing valuable insights into the mechanisms of cell death and potential therapeutic targets for various diseases.
Product Name:
[KO Validated] Bax Rabbit Polyclonal Antibody
SKU:
CAB12009
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bax (NP_620116.1).
BAX (also known as BCL2 Associated X, Bcl-2-Like Protein 4, Bcl2-L-4, BCL2L4) is a member of the BCL2 family of proteins that play a key role in the regulation of apoptosis in higher eukaryotes (https://www.uniprot.org/uniprot/Q07812). BAX comprises 4 Bcl-2 homology domains (BH1-BH4) and a C-terminal transmembrane domain. In healthy mammalian cells, BAX is localized to the cytoplasm through its interaction with the anti-apoptotic BL-2 family members BCL2L1/Bcl-xL . In response to apoptotic stimuli, however, BAX undergoes a conformational change that causes it to translocate to the outer mitochondrial membrane where it initiates the mitochondrial pathway of apoptosis via two potential mechanisms. Firstly, upon translocation to the outer mitochondrial membrane, BAX interacts with the mitochondrial voltage-dependent anion channel (VDAC) leading to the opening of the channel, loss of membrane potential, and the release of cytochrome c from the mitochondrion . The release of cytochrome C into the cytoplasm leads to the activation of Caspase3, initiating apoptosis. Secondly, activated BAX forms homodimers, which then assemble into oligomers on the mitochondrial outer membrane to create pores that permeabilize the mitochondrion leading to the release of cytochrome C.BAX has been shown to be involved in p53-mediated apoptosis. Expression of the human bax gene has been shown to be directly regulated by p53, and the bax promoter contains four motifs with homology to consensus p53-binding sites. Furthermore, p53 directly interacts with BAX to promote its activation.
Purification Method:
Affinity purification
Gene ID:
581
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Immunofluorescence analysis of NIH/3T3 cells using Bax Rabbit pAb (CAB12009) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
