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[KO Validated] Bax Rabbit Polyclonal Antibody (CAB0207)

SKU:
CAB0207
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
  • KO Validated Antibodies 1 Anti-Bax Antibody CAB0207KO Validated
  • KO Validated Antibodies 1 Anti-Bax Antibody CAB0207KO Validated
  • KO Validated Antibodies 1 Anti-Bax Antibody CAB0207KO Validated
  • KO Validated Antibodies 1 Anti-Bax Antibody CAB0207KO Validated
€139 - €419
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Description

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[KO Validated] Bax Rabbit Polyclonal Antibody (CAB0207)

The Bax Polyclonal Antibody (CAB0207) is a crucial tool for researchers studying the Bax protein, a key regulator of apoptosis and cell death. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.Bax is a pro-apoptotic protein that plays a critical role in the intrinsic pathway of apoptosis, promoting cell death under various conditions such as DNA damage or cellular stress. Dysregulation of Bax can have profound effects on cell survival and contribute to the development of diseases such as cancer.With the ability to specifically bind to Bax, this antibody enables researchers to detect and analyze the expression and localization of the protein in different cell types.

Its versatility makes it an ideal tool for investigating the mechanisms of apoptosis and exploring potential therapeutic targets for conditions where aberrant cell death plays a role.Overall, the Bax Polyclonal Antibody (CAB0207) is a valuable resource for scientists conducting research in cell biology, oncology, and drug development, offering insights into the intricate processes governing cell fate and survival.

Product Name:[KO Validated] Bax Rabbit Polyclonal Antibody
SKU:CAB0207
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bax (NP_620116.1).
Sequence:MDGS GEQP RGGG PTSS EQIM KTGA LLLQ GFIQ DRAG RMGG EAPE LALD PVPQ DAST KKLS ECLK RIGD ELDS NMEL QRMI AAVD TDSP REVF FRVA ADMF
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5ug-4ug antibody for 200ug-400ug extracts of whole cells
Synonyms:BCL2 Associated X; Bcl-2-Like Protein 4; Bcl2-L-4; BCL2L4;BAX
Positive Sample:HT-1080,Raji,293T
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Cytoplasm, Mitochondrion membrane, Single-pass membrane protein.
Calculated MW:21kDa
Observed MW:21kDa

BAX (also known as BCL2 Associated X, Bcl-2-Like Protein 4, Bcl2-L-4, BCL2L4) is a member of the BCL2 family of proteins that play a key role in the regulation of apoptosis in higher eukaryotes (https://www.uniprot.org/uniprot/Q07812). BAX comprises 4 Bcl-2 homology domains (BH1-BH4) and a C-terminal transmembrane domain. In healthy mammalian cells, BAX is localized to the cytoplasm through its interaction with the anti-apoptotic BL-2 family members BCL2L1/Bcl-xL . In response to apoptotic stimuli, however, BAX undergoes a conformational change that causes it to translocate to the outer mitochondrial membrane where it initiates the mitochondrial pathway of apoptosis via two potential mechanisms. Firstly, upon translocation to the outer mitochondrial membrane, BAX interacts with the mitochondrial voltage-dependent anion channel (VDAC) leading to the opening of the channel, loss of membrane potential, and the release of cytochrome c from the mitochondrion . The release of cytochrome C into the cytoplasm leads to the activation of Caspase3, initiating apoptosis. Secondly, activated BAX forms homodimers, which then assemble into oligomers on the mitochondrial outer membrane to create pores that permeabilize the mitochondrion leading to the release of cytochrome C.BAX has been shown to be involved in p53-mediated apoptosis. Expression of the human bax gene has been shown to be directly regulated by p53, and the bax promoter contains four motifs with homology to consensus p53-binding sites. Furthermore, p53 directly interacts with BAX to promote its activation.

Purification Method:Affinity purification
Gene ID:581
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and Bax knockout (KO) 293T cells, using [KO Validated] Bax Rabbit pAb (CAB0207) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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