The BARHL1 Monoclonal Antibody (PAC060380) is a valuable tool for researchers studying the BARHL1 protein, a transcription factor involved in neuronal development. This antibody, produced using hybridoma technology, is highly specific for human samples and is suitable for various applications, including immunohistochemistry and flow cytometry.BARHL1 is known to play a critical role in the differentiation and maturation of neurons, making it a key player in neurogenesis and brain development. Dysregulation of BARHL1 expression has been linked to neurodevelopmental disorders and neuronal dysfunction, highlighting the importance of studying this protein in both normal and pathological conditions.
By using the BARHL1 Monoclonal Antibody, researchers can explore the function and regulation of BARHL1 in different cell types and tissues, providing valuable insights into its role in neuronal development and potential implications for neurological disorders. This antibody is a reliable tool for advancing research in neuroscience and understanding the molecular mechanisms underlying brain development.
Western Blot. Positive WB detected in: SH-SY5Y whole cell lysate, U251 whole cell lysate. All lanes: BARHL1 antibody at 3.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 36 kDa. Observed band size: 36 kDa.
IHC image of PACO60380 diluted at 1:400 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
nucleus, RNA polymerase II regulatory region sequence-specific DNA binding, transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding, regulation of transcription from RNA polymerase II promoter
Synonyms:
BarH-like 1 homeobox protein, BARHL1
UniProt Protein Function:
BARHL1: Belongs to the BAR homeobox family.Protein type: Transcription factor; Cell development/differentiation; Motility/polarity/chemotaxisChromosomal Location of Human Ortholog: 9q34Cellular Component: nucleusBiological Process: regulation of transcription from RNA polymerase II promoter
