The BARD1 Polyclonal Antibody (CAB1685) is a powerful tool for researchers studying BARD1, a key protein involved in DNA repair and tumorigenesis. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting BARD1 in human samples, making it ideal for applications such as Western blotting. By binding to the BARD1 protein, this antibody enables researchers to investigate the role of BARD1 in various cellular processes, particularly in the context of cancer biology.BARD1, a critical component of the BRCA1/BARD1 heterodimer, is essential for maintaining genomic stability and preventing the development of tumors.
Dysregulation of BARD1 has been implicated in a variety of cancers, highlighting its potential as a therapeutic target. By elucidating the mechanisms underlying BARD1 function, researchers can gain valuable insights into cancer pathogenesis and potentially identify new strategies for cancer treatment.Overall, the BARD1 Polyclonal Antibody (CAB1685) offers researchers a reliable tool for studying the function of BARD1 in both normal and disease states, paving the way for advancements in cancer research and personalized medicine.
Product Name:
BARD1 Rabbit Polyclonal Antibody
SKU:
CAB1685
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant protein of mouse BARD1
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
BARD1
Positive Sample:
SH-SY5Y,MCF7
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
14kDa/75kDa/84kDa/86kDa
Observed MW:
86kDa
Predicted to enable RNA binding activity; protein heterodimerization activity; and protein homodimerization activity. Predicted to contribute to ubiquitin-protein transferase activity. Predicted to be involved in several processes, including negative regulation of protein export from nucleus; protein K6-linked ubiquitination; and regulation of apoptotic process. Predicted to act upstream of or within DNA repair. Located in nucleus. Is expressed in genitourinary system; limb; and spleen. Used to study breast cancer. Human ortholog(s) of this gene implicated in breast cancer and ovarian cancer. Orthologous to human BARD1 (BRCA1 associated RING domain 1).
Purification Method:
Affinity purification
Gene ID:
12021
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using BARD1 antibody (CAB1685) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
