The BARD1 Polyclonal Antibody (PAC061670) is a vital tool for researchers studying the BARD1 protein, a key player in DNA damage response pathways and a crucial component of the BRCA1 tumor suppressor complex. This antibody is produced in rabbits and is specifically reactive with human samples, making it an essential reagent for Western blot applications. By binding to the BARD1 protein, this antibody allows for precise detection and analysis in various cell types, making it ideal for studies in cancer biology and genomic stability.BARD1 is a critical protein involved in DNA repair and maintenance of genomic integrity, making it a key target for research in cancer biology and therapeutics.
Dysregulation of BARD1 has been implicated in various cancers, suggesting its potential as a prognostic marker or therapeutic target. Understanding the role of BARD1 in DNA repair pathways is essential for developing targeted therapies and personalized treatment strategies for cancer patients. The BARD1 Polyclonal Antibody is a valuable tool for researchers aiming to unravel the complexities of DNA damage response mechanisms and their implications in cancer development and treatment.
Antibody Name:
BARD1 Antibody (PACO61670)
Antibody SKU:
PACO61670
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:5000
Species Reactivity:
Human
Immunogen:
Recombinant Human BRCA1-associated RING domain protein 1 protein (428-777AA)
Western Blot. Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, U87 whole cell lysate, SH-SY5Y whole cell lysate. All lanes: BARD1 antibody at 3.6µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 87, 85, 76, 15 kDa. Observed band size: 87 kDa.
Background:
E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage.
Synonyms:
BRCA1-associated RING domain protein 1, RING-type E3 ubiquitin transferase BARD1, , BARD1
UniProt Protein Function:
UniProt Protein Details:
NCBI Summary:
This gene encodes a protein which interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, it shares homology with the 2 most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes. This protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. This protein may be the target of oncogenic mutations in breast or ovarian cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]
