The BAG3 Rabbit Monoclonal Antibody (CAB4720) is a valuable tool for researchers studying BAG3, a protein involved in cell survival and anti-apoptotic pathways. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blotting, immunohistochemistry, and immunofluorescence applications.BAG3 is known for its role in regulating protein quality control and cell stress response, making it a key player in diseases like cancer, neurodegenerative disorders, and heart disease.
By targeting BAG3 with this monoclonal antibody, researchers can gain insights into the function and expression of BAG3 in various cell types, offering valuable data for studies in cell biology and disease mechanisms.With its high specificity and sensitivity, the BAG3 Rabbit Monoclonal Antibody (CAB4720) enables accurate detection and analysis of BAG3 protein levels, making it an essential tool for unraveling the complex pathways associated with BAG3-mediated cell survival and disease progression.
Product Name:
BAG3 Rabbit Monoclonal Antibody
SKU:
CAB4720
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human BAG3 (O95817).
BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.
Purification Method:
Affinity purification
Gene ID:
9531
Clone Number:
ARC1104
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using BAG3 Rabbit mAb (CAB4720) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
