Bad (Ab-112) Antibody (PACO22892)
- SKU:
- PACO22892
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
Bad (Ab-112) Antibody (PACO22892)
The BAD Antibody (AB-112) is a highly specific and sensitive tool for research involving the protein BAD, a key regulator of apoptosis and cell survival pathways. This polyclonal antibody, produced in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blot applications. By binding to the BAD protein, this antibody enables precise detection and analysis in various cell types, making it an invaluable tool for studies in cell biology and cancer research.BAD, also known as Bcl-2-associated death promoter, is a critical player in cell death mechanisms, influencing processes such as apoptosis and autophagy.
Its central role in determining cell fate and survival makes it a promising target for investigations into cancer, neurodegenerative disorders, and other diseases characterized by dysregulated cell death pathways. Understanding the function of BAD is essential for developing therapeutic strategies that target aberrant cell survival mechanisms and promote healthy cell turnover.
| Antibody Name: | Bad (Ab-112) Antibody (PACO22892) |
| Antibody SKU: | PACO22892 |
| Size: | 100ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:1000, IHC:1:50-1:200 |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Peptide sequence around aa.110~114 (H-S-S-Y-P) derived from Mouse BAD. |
| Form: | Liquid |
| Storage Buffer: | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification Method: | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot analysis of extracts from 293 cells using BAD(Ab-112) Antibody and the same antibody preincubated with blocking peptide. |
 | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD(Ab-112) Antibody(left) or the same antibody preincubated with blocking peptide(right). |
| Background: | The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. |
| Synonyms: | Bbc2 |
| UniProt Protein Function: | BAD: a proapoptotic member of the Bcl-2 family. Displaces Bax from binding to Bcl-2 and Bcl-xL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad inducing the phosphorylation of Bad by Akt and p90RSK. 14-3-3 proteins bind phosphorylated Bad, inhibiting its binding to Bcl-2 and Bcl-xL. Phosphorylation by mitochondria-anchored PKA in the BH3 domain can block the dimerization of Bad and Bcl-xL. |
| UniProt Protein Details: | Protein type:Apoptosis Cellular Component: mitochondrial outer membrane; mitochondrion; membrane; cytoplasm; intracellular; cytosol Molecular Function:protein kinase B binding; protein binding; protein heterodimerization activity; phospholipid binding; caspase activator activity; protein kinase binding; lipid binding; protein phosphatase binding; protein phosphatase 2B binding Biological Process: positive regulation of proteolysis; apoptosis; positive regulation of apoptosis; positive regulation of caspase activity; regulation of caspase activity; cellular process regulating host cell cycle in response to virus; glucose homeostasis; positive regulation of apoptosis by virus; regulation of apoptosis; pore complex biogenesis; positive regulation of glucokinase activity; caspase activation; release of cytochrome c from mitochondria; cytokine and chemokine mediated signaling pathway; suppression by virus of host apoptosis; ADP metabolic process; positive regulation of insulin secretion; ATP metabolic process; glucose catabolic process; cell proliferation; positive regulation of T cell differentiation; induction of apoptosis via death domain receptors; DNA damage response, signal transduction resulting in induction of apoptosis; positive regulation of B cell differentiation; regulation of mitochondrial membrane permeability; positive regulation of epithelial cell proliferation |
| UniProt Code: | Q61337 |
| NCBI GenInfo Identifier: | 2493288 |
| NCBI Gene ID: | 12015 |
| NCBI Accession: | Q61337.1 |
| UniProt Related Accession: | Q61337 |
| Molecular Weight: | |
| NCBI Full Name: | Bcl2-associated agonist of cell death |
| NCBI Synonym Full Names: | BCL2-associated agonist of cell death |
| NCBI Official Symbol: | Bad  |
| NCBI Official Synonym Symbols: | Bbc2; AI325008Â Â |
| NCBI Protein Information: | bcl2-associated agonist of cell death; bcl-2-binding component 6; bcl2 antagonist of cell death; bcl-xL/Bcl-2-associated death promoter |
| UniProt Protein Name: | Bcl2-associated agonist of cell death |
| UniProt Synonym Protein Names: | Bcl-2-binding component 6; Bcl-xL/Bcl-2-associated death promoter; Bcl2 antagonist of cell death |
| Protein Family: | Bcl2-associated agonist of cell death |
| UniProt Gene Name: | Bad  |
| UniProt Entry Name: | BAD_MOUSE |
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