The Bach2 Polyclonal Antibody (PACO60392) is a valuable tool for researchers studying the Bach2 protein, a transcription factor essential for immune regulation and B cell development. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for Western blot applications. By targeting the Bach2 protein, researchers can assess its expression and function in various cell types, providing valuable insights into immunological processes and potential therapeutic targets.Bach2, a key regulator of immune responses, plays a crucial role in maintaining immune balance and preventing autoimmune diseases.
Its dysregulation has been implicated in various immune-related disorders, including cancer and inflammatory conditions. By investigating the role of Bach2 using this antibody, researchers can further elucidate its mechanisms of action and potential implications for disease pathology and treatment strategies. Overall, the Bach2 Polyclonal Antibody is a reliable tool for researchers interested in understanding the complex interplay of immune regulation and disease pathogenesis, offering new avenues for therapeutic development and personalized medicine approaches.
IHC image of PACO60392 diluted at 1:200 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of SH-SY5Y cells with PACO60392 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Transcriptional regulator that acts as repressor or activator (By similarity). Binds to Maf recognition elements (MARE) (By similarity). Plays an important role in coordinating transcription activation and repression by MAFK (By similarity). Induces apoptosis in response to oxidative stress through repression of the antiapoptotic factor HMOX1. Positively regulates the nuclear import of actin (By similarity).
Synonyms:
Transcription regulator protein BACH2 (BTB and CNC homolog 2), BACH2
UniProt Protein Function:
BACH2: Transcriptional regulator that acts as repressor or activator. Binds to Maf recognition elements (MARE). Play important roles in coordinating transcription activation and repression by MAFK. Belongs to the bZIP family. CNC subfamily.Protein type: Transcription factor; DNA-bindingChromosomal Location of Human Ortholog: 6q15Cellular Component: nucleusMolecular Function: transcription factor activity; ubiquitin-protein ligase activityBiological Process: transcription from RNA polymerase II promoter
