The BAAT Polyclonal Antibody (CAB7646) is a valuable tool for researchers studying the BAAT protein, a key enzyme involved in bile acid metabolism. This antibody, generated in rabbits, offers high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the BAAT protein, researchers can detect and analyze its expression in various cell types, making it an essential resource for studies in biochemistry and liver disease research.
BAAT, also known as bile acid-CoA:amino acid N-acyltransferase, plays a crucial role in the formation of bile acids, which are essential for the digestion and absorption of fats. Dysregulation of BAAT expression has been linked to disorders of bile acid metabolism, such as cholestasis and liver diseases. Investigating the function of BAAT is essential for understanding these conditions and developing potential treatments targeting bile acid synthesis pathways.
Product Name:
BAAT Rabbit Polyclonal Antibody
SKU:
CAB7646
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 309-418 of human BAAT (NP_001692.1).
The protein encoded by this gene is a liver enzyme that catalyzes the transfer of C24 bile acids from the acyl-CoA thioester to either glycine or taurine, the second step in the formation of bile acid-amino acid conjugates. The bile acid conjugates then act as a detergent in the gastrointestinal tract, which enhances lipid and fat-soluble vitamin absorption. Defects in this gene are a cause of familial hypercholanemia (FHCA). Two transcript variants encoding the same protein have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
570
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using BAAT Rabbit pAb (CAB7646) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
