The B4GALT5 Polyclonal Antibody (CAB17590) is a valuable tool for researchers studying B4GALT5, a key enzyme involved in the biosynthesis of glycoproteins and glycolipids. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding to the B4GALT5 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential reagent for studies in glycobiology and related fields.B4GALT5 plays a crucial role in glycosylation processes, contributing to the structure and function of numerous proteins and lipids within cells.
Its involvement in glycan biosynthesis makes it a potential target for research on various diseases, such as cancer, congenital disorders of glycosylation, and other metabolic disorders. Understanding the function and regulation of B4GALT5 can provide insights into disease mechanisms and potential therapeutic interventions targeting glycosylation pathways.
Product Name:
B4GALT5 Rabbit Polyclonal Antibody
SKU:
CAB17590
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 60-160 of human B4GALT5 (NP_004767.1).
Sequence:
IGAQ VYEQ VLRS AYAK RNSS VNDS DYPL DLNH SETF LQTT TFLP EDFT YFAN HTCP ERLP SMKG PIDI NMSE IGMD YIHE LFSK DPTI KLGG HWKP SDCM P
This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The function of the enzyme encoded by this gene is not clear. This gene was previously designated as B4GALT4 but was renamed to B4GALT5. In the literature it is also referred to as beta4GalT2.
Purification Method:
Affinity purification
Gene ID:
9334
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using B4GALT5 Rabbit pAb (CAB17590) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
