The B4GALT4 Polyclonal Antibody (CAB14693) is a valuable tool for research involving the B4GALT4 protein, a key enzyme involved in glycosylation processes. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the B4GALT4 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential component for studies in glycosylation pathways and related research areas.B4GALT4, also known as beta-1,4-galactosyltransferase 4, plays a crucial role in the biosynthesis of complex glycoproteins and glycolipids, impacting various cellular functions including cell adhesion, signaling, and immune response modulation.
Research on B4GALT4 is important for understanding its involvement in disease processes such as cancer metastasis, inflammatory disorders, and congenital glycosylation disorders. By elucidating the functions of B4GALT4, researchers can unveil potential therapeutic targets for addressing these conditions and advancing medical treatments.
Product Name:
B4GALT4 Rabbit Polyclonal Antibody
SKU:
CAB14693
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 35-344 of human B4GALT4 (NP_003769.1).
Golgi apparatus, Golgi stack membrane, Single-pass type II membrane protein.
Calculated MW:
40kDa
Observed MW:
40kDa
This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene appears to mainly play a role in glycolipid biosynthesis. Two alternatively spliced transcript variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
8702
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using B4GALT4 Rabbit pAb (CAB14693) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
