The B4GALT3 Polyclonal Antibody (CAB17572) is specifically designed for research involving B4GALT3, a key enzyme involved in the biosynthesis of the carbohydrate antigen Lewis Y. The antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in various applications, including Western blot and immunohistochemistry.B4GALT3, a member of the beta-1,4-galactosyltransferase enzyme family, plays a crucial role in the synthesis of Lewis Y antigen, which is involved in various biological processes, including cell adhesion and signaling.
Dysregulation of B4GALT3 expression has been implicated in various diseases, including cancer and inflammatory conditions.Research utilizing the B4GALT3 Polyclonal Antibody can provide valuable insights into the function and expression of B4GALT3 in different cell types and tissues. This antibody is an essential tool for studying the role of B4GALT3 in various biological processes and disease states, making it an ideal choice for researchers in the fields of glycobiology, cancer biology, and immunology.
Product Name:
B4GALT3 Rabbit Polyclonal Antibody
SKU:
CAB17572
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 40-150 of human B4GALT3 (NP_003770.1).
Golgi apparatus, Golgi stack membrane, Single-pass type II membrane protein.
Calculated MW:
44kDa
Observed MW:
44kDa
This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. This gene encodes an enzyme that may be mainly involved in the synthesis of the first N-acetyllactosamine unit of poly-N-acetyllactosamine chains. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
8703
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using B4GALT3 Rabbit pAb (CAB17572) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
