The B4GALT2 Polyclonal Antibody (CAB17573) is a high-quality antibody designed for research involving B4GALT2, a key enzyme involved in glycoprotein biosynthesis. The antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blot and immunohistochemistry.B4GALT2, also known as beta-1,4-galactosyltransferase 2, plays a crucial role in the biosynthesis of glycoproteins, which are essential for various cellular functions, including cell adhesion and signaling.
Dysregulation of B4GALT2 has been implicated in various diseases, including cancer and congenital disorders.This antibody enables the detection and analysis of B4GALT2 protein in different cell types, making it an ideal tool for studying glycoprotein biosynthesis and its implications in disease processes. Understanding the function of B4GALT2 could potentially lead to the development of targeted therapies for diseases associated with glycoprotein abnormalities.
Product Name:
B4GALT2 Rabbit Polyclonal Antibody
SKU:
CAB17573
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 155-271 of human B4GALT2 (NP_085076.2).
U-87MG,PC-3,OVCAR3,HT-29,Mouse brain,Mouse skeletal muscle,Mouse large intestine,Rat brain
Conjugate:
Unconjugated
Cellular Localization:
Golgi apparatus, nucleoplasm
Calculated MW:
42kDa
Observed MW:
42kDa
This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene synthesizes N-acetyllactosamine in glycolipids and glycoproteins. Its substrate specificity is affected by alpha-lactalbumin but it is not expressed in lactating mammary tissue. Three transcript variants encoding two different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
8704
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using B4GALT2 Rabbit pAb (CAB17573) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
