The B4GALT1 Polyclonal Antibody is a vital tool for researchers studying B4GALT1, an enzyme involved in the glycosylation of proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is specifically validated for use in Western blot applications. By binding to the B4GALT1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential tool for investigations in glycobiology and related fields.B4GALT1 plays a crucial role in protein glycosylation, a post-translational modification that influences protein function and stability. Dysregulation of this enzyme has been linked to various diseases, including cancer and genetic disorders.
By studying B4GALT1 with this antibody, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets.For researchers interested in unraveling the complexities of protein glycosylation and its impact on human health, the B4GALT1 Polyclonal Antibody offers a reliable and effective solution for their experimental needs. With its ability to accurately detect and quantify B4GALT1 expression, this antibody enables detailed investigations that could lead to novel discoveries in the fields of biochemistry and molecular biology.
Product Name:
B4GALT1 Polyclonal Antibody
SKU:
CAB21847
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 50-215 of human B4GALT1 (NP_001488.2).
Cell membrane, Cell projection, Cell surface, Golgi apparatus, Golgi stack membrane, Secreted, Single-pass type II membrane protein, filopodium.
Calculated MW:
44kDa
Observed MW:
Refer to figures
This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. This gene is unique among the beta4GalT genes because it encodes an enzyme that participates both in glycoconjugate and lactose biosynthesis. For the first activity, the enzyme adds galactose to N-acetylglucosamine residues that are either monosaccharides or the nonreducing ends of glycoprotein carbohydrate chains. The second activity is restricted to lactating mammary tissues where the enzyme forms a heterodimer with alpha-lactalbumin to catalyze UDP-galactose + D-glucose <=> UDP + lactose. The two enzymatic forms result from alternate transcription initiation sites and post-translational processing. Two transcripts, which differ only at the 5' end, with approximate lengths of 4.1 kb and 3.9 kb encode the same protein. The longer transcript encodes the type II membrane-bound, trans-Golgi resident protein involved in glycoconjugate biosynthesis. The shorter transcript encodes a protein which is cleaved to form the soluble lactose synthase.
Purification Method:
Affinity purification
Gene ID:
2683
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using B4GALT1 Rabbit pAb (CAB21847) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
