Anti-B3GNT5 Antibody (CAB14428)
- SKU:
- CAB14428
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
Anti-B3GNT5 Antibody (CAB14428)
The B3GNT5 Polyclonal Antibody (CAB14428) is a crucial tool for researchers studying B3GNT5, a glycosyltransferase enzyme involved in the biosynthesis of complex gangliosides. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.B3GNT5 plays a significant role in the regulation of cellular processes such as cell growth, differentiation, and adhesion, making it a promising target for research in the fields of cancer biology and neurobiology.
By binding specifically to the B3GNT5 protein, this antibody allows for precise detection and analysis in a variety of cell types.Understanding the function and regulation of B3GNT5 is essential for elucidating its role in health and disease, particularly in the context of cancer progression and neurological disorders. The B3GNT5 Polyclonal Antibody is a valuable tool for researchers seeking to unravel the complexities of glycosylation pathways and their impact on cellular physiology.
| Antibody Name: | Anti-B3GNT5 Antibody |
| Antibody SKU: | CAB14428 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 36-378 of human B3GNT5 (NP_114436.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HT-29, A-549 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 36-378 of human B3GNT5 (NP_114436.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | DNHI VSHM KSYS YRYL INSY DFVN DTLS LKHT SAGP RYQY LINH KEKC QAQD VLLL LFVK TAPE NYDR RSGI RRTW GNEN YVRS QLNA NIKT LFAL GTPN PLEG EELQ RKLA WEDQ RYND IIQQ DFVD SFYN LTLK LLMQ FSWA NTYC PHAK FLMT ADDD IFIH MPNL IEYL QSLE QIGV QDFW IGRV HRGA PPIR DKSS KYYV SYEM YQWP AYPD YTAG AAYV ISGD VAAK VYEA SQTL NSSL YIDD VFMG LCAN KIGI VPQD HVFF SGEG KTPY HPCI YEKM MTSH GHLE DLQD LWKN ATDP KVKT ISKG FFGQ IYCR LMKI ILLC KISY VDTY PCRA AFI |
| Gene ID: | 84002 |
| Uniprot: | Q9BYG0 |
| Cellular Location: | Golgi apparatus membrane, Single-pass type II membrane protein |
| Calculated MW: | 44kDa |
| Observed MW: | 44kDa |
| Synonyms: | B3GNT5, B3GN-T5 |
| Background: | This gene encodes a member of the beta-1, 3-N-acetylglucosaminyltransferase family. This enzyme is a type II membrane protein. It exhibits strong activity to transfer GlcNAc to glycolipid substrates and is identified as the most likely candidate for lactotriaosylceramide synthase. This enzyme is essential for the expression of Lewis X epitopes on glycolipids. |
| UniProt Protein Function: | B3GNT5: Beta-1,3-N-acetylglucosaminyltransferase that plays a key role in the synthesis of lacto- or neolacto-series carbohydrate chains on glycolipids, notably by participating in biosynthesis of HNK-1 and Lewis X carbohydrate structures. Has strong activity toward lactosylceramide (LacCer) and neolactotetraosylceramide (nLc(4)Cer; paragloboside), resulting in the synthesis of Lc(3)Cer and neolactopentaosylceramide (nLc(5)Cer), respectively. Probably plays a central role in regulating neolacto-series glycolipid synthesis during embryonic development. Belongs to the glycosyltransferase 31 family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Transferase; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; EC 2.4.1.206 Chromosomal Location of Human Ortholog: 3q28 Cellular Component: Golgi membrane; integral to membrane; intracellular Molecular Function:lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase activity; galactosyltransferase activity; beta-galactosyl-N-acetylglucosaminylgalactosylglucosyl-ceramide beta-1,3-acetylglucosaminyltransferase activity Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; central nervous system development; O-glycan processing; glycolipid biosynthetic process; protein amino acid glycosylation; post-translational protein modification |
| NCBI Summary: | This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II membrane protein. It exhibits strong activity to transfer GlcNAc to glycolipid substrates and is identified as the most likely candidate for lactotriaosylceramide synthase. This enzyme is essential for the expression of Lewis X epitopes on glycolipids. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9BYG0 |
| NCBI GenInfo Identifier: | 74733473 |
| NCBI Gene ID: | 84002 |
| NCBI Accession: | Q9BYG0.1 |
| UniProt Secondary Accession: | Q9BYG0,Q59FE3, Q7L9Z5, Q8WWP9, D3DNS5, |
| UniProt Related Accession: | Q9BYG0 |
| Molecular Weight: | 378 |
| NCBI Full Name: | Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase |
| NCBI Synonym Full Names: | UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 |
| NCBI Official Symbol: | B3GNT5Â Â |
| NCBI Official Synonym Symbols: | B3GN-T5; beta3Gn-T5Â Â |
| NCBI Protein Information: | lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase; BGnT-5; lc3 synthase; beta-1,3-Gn-T5; lc(3)Cer synthase; lactotriaosylceramide synthase; beta-1,3-N-acetylglucosaminyltransferase 5; beta-1,3-N-acetylglucosaminyltransferase bGnT-5; beta 1,3 N-acetyglucosaminyltransferase Lc3 synthase; UDP-GlcNAc:beta-Gal beta-1,3-N-acetylglucosaminyltransferase 5 |
| UniProt Protein Name: | Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase |
| UniProt Synonym Protein Names: | Lactotriaosylceramide synthase; Lc(3)Cer synthase; Lc3 synthase; UDP-GlcNAc:beta-Gal beta-1,3-N-acetylglucosaminyltransferase 5; BGnT-5; Beta-1,3-Gn-T5; Beta-1,3-N-acetylglucosaminyltransferase 5; Beta3Gn-T5 |
| Protein Family: | Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase |
| UniProt Gene Name: | B3GNT5Â Â |
| UniProt Entry Name: | B3GN5_HUMAN |
 | Western blot analysis of extracts of various cell lines, using B3GNT5 antibody (CAB14428) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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