The B3GALT4 Polyclonal Antibody (CAB15341) is a powerful tool for researchers studying B3GALT4, a key enzyme involved in the biosynthesis of glycosphingolipids. This antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in various applications including Western blot and immunofluorescence.B3GALT4 is an essential enzyme in glycosylation pathways, playing a critical role in the synthesis of complex glycolipids that are involved in various cellular processes such as cell adhesion, signal transduction, and immune response.
Dysregulation of B3GALT4 has been linked to various diseases, making it an important target for research in cancer biology, immunology, and metabolic disorders.By targeting the B3GALT4 protein, researchers can investigate its function and role in different cellular pathways, providing valuable insights into disease mechanisms and potential therapeutic strategies. The B3GALT4 Polyclonal Antibody is an essential tool for researchers seeking to expand their understanding of glycosylation processes and their impact on human health.
Product Name:
B3GALT4 Rabbit Polyclonal Antibody
SKU:
CAB15341
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 80-270 of human B3GALT4 (NP_003773.1).
Golgi apparatus membrane, Single-pass type II membrane protein.
Calculated MW:
42kDa
Observed MW:
42kDa
This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5). This gene is oriented telomere to centromere in close proximity to the ribosomal protein S18 gene. The functionality of the encoded protein is limited to ganglioseries glycolipid biosynthesis.
Purification Method:
Affinity purification
Gene ID:
8705
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse heart, using B3GALT4 Rabbit pAb (CAB15341) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
