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B-Raf Polyclonal Antibody (CAB21612)

SKU:
CAB21612
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
€129 - €359
Frequently bought together:

Description

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B-Raf Polyclonal Antibody (CAB21612)

The B-RAF Polyclonal Antibody (CAB21612) is a valuable tool for researchers studying the B-RAF protein, a key player in cell signaling pathways and cancer development. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications.B-RAF is a serine/threonine protein kinase that is mutated in various cancers, making it a promising target for cancer therapy. By detecting and analyzing B-RAF protein levels in different cell types, this antibody is essential for investigating the role of B-RAF in cancer progression and drug resistance mechanisms.

Furthermore, understanding the function of B-RAF is critical for developing targeted therapies and personalized medicine strategies for cancer treatment. With its specificity and sensitivity, the B-RAF Polyclonal Antibody is a valuable asset for researchers in the fields of oncology and cell signaling research.

Product Name:B-Raf Polyclonal Antibody
SKU:CAB21612
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 350-449 of human B-Raf (NP_004324.2).
Sequence:DEDH RNQF GQRD RSSS APNV HINT IEPV NIDD LIRD QGFR GDGG STTG LSAT PPAS LPGS LTNV KALQ KSPG PQRE RKSS SSSE DRNR MKTL GRRD SSDD
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:NS7; B-raf; BRAF1; RAFB1; B-RAF1; BRAF-1; B-Raf
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm, Nucleus.
Calculated MW:84kDa
Observed MW:Refer to figures

This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome.

Purification Method:Affinity purification
Gene ID:673
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using B-Raf antibody (CAB21612) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

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