The AZIN1 Polyclonal Antibody (CAB4747) is specifically designed for research involving AZIN1, a protein involved in the regulation of polyamine metabolism. This antibody, raised in rabbits, is highly reactive with human samples and is validated for use in Western blot applications. It binds specifically to the AZIN1 protein, allowing for detection and analysis in various cell types.AZIN1 plays a crucial role in regulating polyamine levels, which are essential for cell growth and proliferation. Dysregulation of polyamine metabolism has been implicated in various diseases, including cancer and neurological disorders.
Studying the function of AZIN1 can provide valuable insights into these diseases and potentially lead to the development of targeted therapies.Overall, the AZIN1 Polyclonal Antibody is a valuable tool for researchers studying polyamine metabolism and its role in health and disease. Its high reactivity and specificity make it ideal for use in a variety of research applications, making it a valuable asset for laboratories focusing on cancer, neuroscience, and metabolic disorders.
Product Name:
AZIN1 Rabbit Polyclonal Antibody
SKU:
CAB4747
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 289-448 of human AZIN1 (NP_056962.2).
The protein encoded by this gene belongs to the antizyme inhibitor family, which plays a role in cell growth and proliferation by maintaining polyamine homeostasis within the cell. Antizyme inhibitors are homologs of ornithine decarboxylase (ODC, the key enzyme in polyamine biosynthesis) that have lost the ability to decarboxylase ornithine; however, retain the ability to bind to antizymes. Antizymes negatively regulate intracellular polyamine levels by binding to ODC and targeting it for degradation, as well as by inhibiting polyamine uptake. Antizyme inhibitors function as positive regulators of polyamine levels by sequestering antizymes and neutralizing their effect. This gene encodes antizyme inhibitor 1, the first member of this gene family that is ubiquitously expressed, and is localized in the nucleus and cytoplasm. Overexpression of antizyme inhibitor 1 gene has been associated with increased proliferation, cellular transformation and tumorigenesis. Gene knockout studies showed that homozygous mutant mice lacking functional antizyme inhibitor 1 gene died at birth with abnormal liver morphology. RNA editing of this gene, predominantly in the liver tissue, has been linked to the progression of hepatocellular carcinoma. Alternatively spliced transcript variants have been described for this gene.
Purification Method:
Affinity purification
Gene ID:
51582
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using AZIN1 Rabbit pAb (CAB4747) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
