The Axin1 Polyclonal Antibody (CAB16019) is a valuable tool for research involving Axin1, a key regulator of the Wnt signaling pathway. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to Axin1, this antibody allows for the detection and analysis of Axin1 protein levels in various cell types, making it ideal for studies in developmental biology, cancer research, and regenerative medicine.Axin1 is a crucial component of the destruction complex that regulates the levels of beta-catenin, a key player in the Wnt signaling pathway.
Dysregulation of the Wnt pathway has been implicated in various diseases, including cancer, developmental disorders, and neurodegenerative diseases. By studying Axin1, researchers can gain insights into the mechanisms by which the Wnt pathway is regulated and develop potential therapeutic strategies for targeting this pathway in disease treatment.Overall, the Axin1 Polyclonal Antibody (CAB16019) offers researchers a reliable tool for studying Axin1 and its role in the Wnt signaling pathway, providing valuable insights into important biological processes and potential therapeutic targets for a wide range of diseases.
Product Name:
AXIN1 Rabbit Polyclonal Antibody
SKU:
CAB16019
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-351 of human AXIN1 (NP_851393.1).
This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
8312
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using AXIN1 Rabbit pAb (CAB16019) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
