Anti-AXIN2 Antibody (CAB2513)
- SKU:
- CAB2513
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
Anti-AXIN2 Antibody (CAB2513)
The Axin-2 Polyclonal Antibody (CAB2513) offered by AssayGenie is a valuable tool for research on Axin-2, a key regulator in the Wnt signaling pathway. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the Axin-2 protein, this antibody allows for the detection and analysis of Axin-2 expression in various cell types.Axin-2 is a crucial component in the Wnt signaling pathway, playing a role in the regulation of cell proliferation, differentiation, and migration. Dysregulation of this pathway has been implicated in various diseases, including cancer and developmental disorders.
Therefore, studying Axin-2 expression and function is essential for understanding the mechanisms underlying these diseases and for developing targeted therapies.With its high reactivity and specificity, the Axin-2 Polyclonal Antibody (CAB2513) is an excellent choice for researchers in the fields of molecular biology, cancer biology, and developmental biology. Its ability to detect Axin-2 in human samples makes it a valuable tool for investigating the role of this protein in disease pathology and for identifying potential therapeutic targets.
| Antibody Name: | Anti-AXIN2 Antibody |
| Antibody SKU: | CAB2513 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 650-750 of human AXIN2 (NP_004646.3). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, SW480, Mouse brain, Rat brain, Rat thymus |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 650-750 of human AXIN2 (NP_004646.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RSSP GERA SRHH LWGG NSGH PRTT PRAH LFTQ DPAM PPLT PPNT LAQL EEAC RRLA EVSK PPKQ RCCV ASQQ RDRN HSAT VQTG ATPF SNPS LAPE DHKE P |
| Gene ID: | 8313 |
| Uniprot: | Q9Y2T1 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 93kDa |
| Observed MW: | 93kDa |
| Synonyms: | AXIN2, AXIL, ODCRCS, axin-2 |
| Background: | The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. |
| UniProt Protein Function: | axin 2: is a negative regulator of the Wnt pathway, which is critical in stem cell signaling, morphogenesis, the mesenchymal-epithelial transition, and many cancers. Axin functions as a tumor suppressor. Probably facilitates the phosphorylation of beta-catenin and APC by GSK3B, leading to their ubiquitination and subsequent proteolysis. Is downregulated during progression of esophageal squamous cell carcinoma. Axin1/2 mediate cross-talk between TGF-beta and Wnt signaling pathways. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 17q24.1 Cellular Component: nucleoplasm; microtubule cytoskeleton; Golgi apparatus; centrosome; cytoplasmic microtubule; cytoplasm; cytoplasmic membrane-bound vesicle; postsynaptic density; plasma membrane; cell cortex; cytosol; nucleus; beta-catenin destruction complex Molecular Function:protein binding; enzyme binding; ubiquitin protein ligase binding; beta-catenin binding; protein kinase binding; GTPase activator activity Biological Process: odontogenesis; negative regulation of cell proliferation; cell proliferation; intramembranous ossification; regulation of mismatch repair; mRNA stabilization; dorsal/ventral axis specification; negative regulation of osteoblast differentiation; positive regulation of protein amino acid phosphorylation; maintenance of DNA repeat elements; bone mineralization; positive regulation of GTPase activity Disease: Oligodontia-colorectal Cancer Syndrome |
| NCBI Summary: | The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9Y2T1 |
| NCBI GenInfo Identifier: | 12643949 |
| NCBI Gene ID: | 8313 |
| NCBI Accession: | Q9Y2T1.1 |
| UniProt Secondary Accession: | Q9Y2T1,Q3MJ88, Q9H3M6, Q9UH84, |
| UniProt Related Accession: | Q9Y2T1 |
| Molecular Weight: | 843 |
| NCBI Full Name: | Axin-2 |
| NCBI Synonym Full Names: | axin 2 |
| NCBI Official Symbol: | AXIN2Â Â |
| NCBI Official Synonym Symbols: | AXIL; ODCRCSÂ Â |
| NCBI Protein Information: | axin-2; conductin; axin-like protein; axis inhibition protein 2 |
| UniProt Protein Name: | Axin-2 |
| UniProt Synonym Protein Names: | Axin-like protein; Axil; Axis inhibition protein 2; Conductin |
| Protein Family: | Axin |
| UniProt Gene Name: | AXIN2Â Â |
| UniProt Entry Name: | AXIN2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using AXIN2 antibody (CAB2513) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded rat brain using AXIN2 antibody (CAB2513) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spinal cord using AXIN2 antibody (CAB2513) at dilution of 1:100 (40x lens). |
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