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Anti-AXIN2 Antibody (CAB2513)

SKU:
CAB2513
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism
  • Metabolism Antibodies 2 Anti-AXIN2 Antibody CAB2513
  • Metabolism Antibodies 2 Anti-AXIN2 Antibody CAB2513
  • Metabolism Antibodies 2 Anti-AXIN2 Antibody CAB2513
€139 - €419
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Description

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Antibody Name:Anti-AXIN2 Antibody
Antibody SKU:CAB2513
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 650-750 of human AXIN2 (NP_004646.3).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, SW480, Mouse brain, Rat brain, Rat thymus
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 650-750 of human AXIN2 (NP_004646.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RSSP GERA SRHH LWGG NSGH PRTT PRAH LFTQ DPAM PPLT PPNT LAQL EEAC RRLA EVSK PPKQ RCCV ASQQ RDRN HSAT VQTG ATPF SNPS LAPE DHKE P
Gene ID:8313
Uniprot:Q9Y2T1
Cellular Location:Cytoplasm
Calculated MW:93kDa
Observed MW:93kDa
Synonyms:AXIN2, AXIL, ODCRCS, axin-2
Background:The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair.
UniProt Protein Function:axin 2: is a negative regulator of the Wnt pathway, which is critical in stem cell signaling, morphogenesis, the mesenchymal-epithelial transition, and many cancers. Axin functions as a tumor suppressor. Probably facilitates the phosphorylation of beta-catenin and APC by GSK3B, leading to their ubiquitination and subsequent proteolysis. Is downregulated during progression of esophageal squamous cell carcinoma. Axin1/2 mediate cross-talk between TGF-beta and Wnt signaling pathways.
UniProt Protein Details:

Protein type:Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q24.1

Cellular Component: nucleoplasm; microtubule cytoskeleton; Golgi apparatus; centrosome; cytoplasmic microtubule; cytoplasm; cytoplasmic membrane-bound vesicle; postsynaptic density; plasma membrane; cell cortex; cytosol; nucleus; beta-catenin destruction complex

Molecular Function:protein binding; enzyme binding; ubiquitin protein ligase binding; beta-catenin binding; protein kinase binding; GTPase activator activity

Biological Process: odontogenesis; negative regulation of cell proliferation; cell proliferation; intramembranous ossification; regulation of mismatch repair; mRNA stabilization; dorsal/ventral axis specification; negative regulation of osteoblast differentiation; positive regulation of protein amino acid phosphorylation; maintenance of DNA repeat elements; bone mineralization; positive regulation of GTPase activity

Disease: Oligodontia-colorectal Cancer Syndrome

NCBI Summary:The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. [provided by RefSeq, Jul 2008]
UniProt Code:Q9Y2T1
NCBI GenInfo Identifier:12643949
NCBI Gene ID:8313
NCBI Accession:Q9Y2T1.1
UniProt Secondary Accession:Q9Y2T1,Q3MJ88, Q9H3M6, Q9UH84,
UniProt Related Accession:Q9Y2T1
Molecular Weight:843
NCBI Full Name:Axin-2
NCBI Synonym Full Names:axin 2
NCBI Official Symbol:AXIN2  
NCBI Official Synonym Symbols:AXIL; ODCRCS  
NCBI Protein Information:axin-2; conductin; axin-like protein; axis inhibition protein 2
UniProt Protein Name:Axin-2
UniProt Synonym Protein Names:Axin-like protein; Axil; Axis inhibition protein 2; Conductin
Protein Family:Axin
UniProt Gene Name:AXIN2  
UniProt Entry Name:AXIN2_HUMAN
Anti-AXIN2 Antibody (CAB2513)Western blot analysis of extracts of various cell lines, using AXIN2 antibody (CAB2513) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-AXIN2 Antibody (CAB2513)Immunohistochemistry of paraffin-embedded rat brain using AXIN2 antibody (CAB2513) at dilution of 1:100 (40x lens).
Anti-AXIN2 Antibody (CAB2513)Immunohistochemistry of paraffin-embedded mouse spinal cord using AXIN2 antibody (CAB2513) at dilution of 1:100 (40x lens).

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