The Autophagy Protein 5 Polyclonal Antibody (CAB11427) is a vital tool for researchers studying autophagy, a cellular process essential for maintaining cellular homeostasis and promoting cell survival. This antibody, produced in rabbits, is highly specific to autophagy protein 5 (ATG5) and has been validated for use in Western blot applications.ATG5 is a key player in the autophagy pathway, responsible for the elongation of the isolation membrane and formation of autophagosomes. Dysregulation of the autophagy process has been implicated in various diseases, including cancer, neurodegenerative disorders, and metabolic syndromes. Therefore, studying ATG5 and its role in autophagy regulation is crucial for understanding these conditions and developing potential therapeutic strategies.
With its high reactivity and specificity for ATG5, the Autophagy Protein 5 Polyclonal Antibody (CAB11427) is an invaluable tool for researchers investigating the molecular mechanisms of autophagy and its implications in health and disease. Whether exploring the role of autophagy in cancer development or neurodegenerative diseases, this antibody enables precise detection and analysis of ATG5 in a variety of cell types, making it ideal for a wide range of research applications in cell biology and biomedicine.
Product Name:
ATG5 Rabbit Polyclonal Antibody
SKU:
CAB11427
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 28-275 of human ATG5 (NP_004840.1).
The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
9474
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from SH-SY5Y cells, using ATG5 Rabbit pAb (CAB11427) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
