The Autophagy Protein 5 Polyclonal Antibody (CAB0203) is a valuable tool for researchers studying autophagy, a cellular process essential for maintaining homeostasis by degrading and recycling damaged organelles and proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.Autophagy Protein 5 (ATG5) is a key regulator in the autophagy pathway, essential for the formation of autophagosomes and the initiation of autophagy. Dysregulation of autophagy has been implicated in various diseases such as cancer, neurodegenerative disorders, and inflammatory conditions.
By targeting ATG5 with this antibody, researchers can analyze the role of autophagy in these diseases and explore potential therapeutic interventions.Understanding the molecular mechanisms of autophagy, particularly the function of ATG5, is crucial for developing targeted therapies that modulate autophagy to treat a range of human diseases. The Autophagy Protein 5 Polyclonal Antibody (CAB0203) provides researchers with a specific and reliable tool to advance their studies in autophagy and its implications for human health.
Product Name:
ATG5 Rabbit Polyclonal Antibody
SKU:
CAB0203
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human ATG5 (NP_004840.1).
The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
9474
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ATG5 Rabbit pAb (CAB0203) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
