The Aurora Kinase A Polyclonal Antibody (CAB2121) is a vital tool for researchers studying Aurora Kinase A, a key protein involved in cell cycle regulation and mitosis. This polyclonal antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blotting.Aurora Kinase A is a crucial player in cell division, functioning to ensure accurate chromosome segregation and proper cell division. Dysregulation of Aurora Kinase A has been linked to cancer development and progression, making it an attractive target for cancer research and therapy development.
With the ability to specifically bind to Aurora Kinase A, this antibody enables precise detection and analysis of the protein in various cell types, providing valuable insights into its role in cell cycle progression and potential therapeutic applications in cancer treatment. Researchers in the fields of cell biology, oncology, and drug discovery will benefit from the reliable performance and specificity of this Aurora Kinase A Polyclonal Antibody in their research endeavors.
Product Name:
AURKA Rabbit Polyclonal Antibody
SKU:
CAB2121
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 301-403 of human AURKA (NP_003591.2).
AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; PPP1R47; AURKA
Positive Sample:
HeLa
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, centriole, centrosome, cilium basal body, cytoskeleton, microtubule organizing center, spindle pole.
Calculated MW:
46kDa
Observed MW:
46kDa
The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
6790
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of HeLa cells, using AURKA Rabbit pAb (CAB2121) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
