null

Phospho-AURKA-T288 Rabbit Polyclonal Antibody (CABP0523)

SKU:
CABP0523
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
$503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-AURKA-T288 Rabbit Polyclonal Antibody
SKU:CABP0523
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around T288 of human AURKA (NP_003591.2).
Sequence:RTTL C
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; PPP1R47; Phospho-AURKA-T288
Positive Sample:HeLa,HT-29
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, centriole, centrosome, cilium basal body, cytoskeleton, microtubule organizing center, spindle pole.
Calculated MW:46kDa
Observed MW:48kDa

The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene.

Purification Method:Affinity purification
Gene ID:6790
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of HeLa and HT-29 cells, using Phospho-AURKA-T288 antibody (CABP0523) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours.HeLa cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Related Products