The Aurora Kinase A Polyclonal Antibody (CABP0523) is a valuable tool for researchers studying the Aurora Kinase A protein, a key regulator of cell division and chromosome segregation. This antibody, produced in rabbits, is highly specific for human samples and is validated for use in Western blot and immunohistochemistry applications.Aurora Kinase A plays a crucial role in mitosis, ensuring proper alignment and separation of chromosomes during cell division. Dysregulation of Aurora Kinase A has been implicated in various cancers, making it a promising target for cancer therapy development.
This antibody allows for precise detection and analysis of Aurora Kinase A levels in different cell types, providing valuable insights into its function and potential as a therapeutic target.Overall, the Aurora Kinase A Polyclonal Antibody (CABP0523) is a reliable tool for researchers in the fields of cell biology and cancer research, enabling the study of Aurora Kinase A and its role in cell division and cancer progression.
Product Name:
Phospho-AURKA-T288 Rabbit Polyclonal Antibody
SKU:
CABP0523
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around T288 of human AURKA (NP_003591.2).
Cytoplasm, centriole, centrosome, cilium basal body, cytoskeleton, microtubule organizing center, spindle pole.
Calculated MW:
46kDa
Observed MW:
48kDa
The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
6790
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of HeLa and HT-29 cells, using Phospho-AURKA-T288 antibody (CABP0523) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours.HeLa cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
