The AURKA Polyclonal Antibody (CAB15728) is a valuable tool for researchers studying the AURKA protein, a key regulator of cell division and spindle assembly. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blotting applications. By binding to the AURKA protein, this antibody enables precise detection and analysis in various cell types, making it ideal for investigations in the fields of cell biology and cancer research.AURKA, also known as Aurora kinase A, is crucial for controlling chromosome segregation and ensuring accurate cell division.
Dysregulation of AURKA has been implicated in various diseases, particularly cancer, where overexpression of the protein can lead to uncontrolled cell proliferation. Understanding the functions and mechanisms of AURKA is essential for developing targeted therapies that can disrupt cell cycle progression in cancer cells - making this antibody a valuable tool for unlocking the potential of AURKA as a therapeutic target.
Product Name:
AURKA Rabbit Polyclonal Antibody
SKU:
CAB15728
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human AURKA (NP_940837.1).
AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; PPP1R47; AURKA
Positive Sample:
HeLa,MCF7,HepG2
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, centriole, centrosome, cilium basal body, cytoskeleton, microtubule organizing center, spindle pole.
Calculated MW:
46kDa
Observed MW:
45kDa
The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
6790
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using AURKA antibody (CAB15728) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
