The AUH Polyclonal Antibody (CAB7424) is a valuable tool for researchers investigating AUH, a protein involved in lipid metabolism and mitochondrial function. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the AUH protein, this antibody enables precise detection and analysis in a variety of cell types, making it an ideal choice for studies in biochemistry and mitochondrial research.AUH, also known as AU RNA binding methylglutaconyl-CoA hydratase, plays a crucial role in the breakdown of fatty acids and energy production within the mitochondria.
Dysregulation of AUH has been linked to metabolic disorders and mitochondrial dysfunction, making it a promising target for therapeutic interventions in conditions such as fatty acid oxidation disorders and mitochondrial diseases. Understanding the function and regulation of AUH is essential for developing targeted treatments and furthering our knowledge of metabolic pathways.
Product Name:
AUH Rabbit Polyclonal Antibody
SKU:
CAB7424
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 68-339 of human AUH (NP_001689.1).
This gene encodes bifunctional mitochondrial protein that has both RNA-binding and hydratase activities. The encoded protein is a methylglutaconyl-CoA hydratase that catalyzes the hydration of 3-methylglutaconyl-CoA to 3-hydroxy-3-methyl-glutaryl-CoA, a critical step in the leucine degradation pathway. This protein also binds AU-rich elements (AREs) found in the 3' UTRs of rapidly decaying mRNAs including c-fos, c-myc and granulocyte/ macrophage colony stimulating factor. ARE elements are involved in directing RNA to rapid degradation and deadenylation. This protein is localizes to the mitochondrial matrix and the inner mitochondrial membrane and may be involved in mitochondrial protein synthesis. Mutations in this gene are the cause of 3-methylglutaconic aciduria, type I. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
549
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of HepG2, using AUH Rabbit pAb (CAB7424) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
