The ATRIP Polyclonal Antibody (CAB5041) is a valuable tool for researchers studying the ATRIP protein, an essential component of the ATR (ataxia telangiectasia and Rad3-related protein) signaling pathway involved in DNA damage response. This antibody is produced in rabbits and exhibits high reactivity with human samples, making it suitable for use in Western blot analyses.ATRIP is crucial for the activation of ATR kinase in response to DNA damage, playing a key role in the maintenance of genomic stability. Dysregulation of the ATR pathway has been linked to various human diseases, including cancer and neurodegenerative disorders.
By targeting ATRIP with this polyclonal antibody, researchers can gain insights into the mechanisms underlying DNA damage repair and potential therapeutic strategies for diseases associated with genomic instability.The ATRIP Polyclonal Antibody (CAB5041) is an essential tool for investigating the function of ATRIP in cellular processes such as DNA repair, cell cycle checkpoint activation, and apoptosis. Its specificity and reliability in detecting ATRIP make it a valuable asset for research in the fields of oncology, genetics, and molecular biology.
Product Name:
ATRIP Rabbit Polyclonal Antibody
SKU:
CAB5041
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ATRIP (NP_569055.1).
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
ATRIP
Positive Sample:
Jurkat,HeLa
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
86kDa
Observed MW:
100kDa/113kDa
This gene encodes an essential component of the DNA damage checkpoint. The encoded protein binds to single-stranded DNA coated with replication protein A. The protein also interacts with the ataxia telangiectasia and Rad3 related protein kinase, resulting in its accumulation at intranuclear foci induced by DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
84126
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using ATRIP Rabbit pAb (CAB5041) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
