The ATP7B Polyclonal Antibody (CAB5676) is a valuable tool for researchers studying ATP7B, a transmembrane copper-transporting P-type ATPase involved in copper homeostasis. This antibody, raised in rabbits, is highly specific for human samples and is validated for use in various applications, including Western blotting. By binding to the ATP7B protein, this antibody enables the detection and analysis of ATP7B expression in different cell types, making it ideal for studies in biochemistry and neuroscience.ATP7B plays a crucial role in copper transport and metabolism, with mutations in the ATP7B gene leading to disorders such as Wilson's disease, a hereditary disorder resulting in copper accumulation and toxicity.
Understanding the function and regulation of ATP7B is essential for developing therapies for copper-related disorders and exploring the role of copper in various physiological processes.Overall, the ATP7B Polyclonal Antibody is a valuable resource for researchers investigating the role of ATP7B in copper homeostasis and related disorders, offering high specificity and sensitivity for accurate detection and analysis in experimental settings.
Product Name:
ATP7B Rabbit Polyclonal Antibody
SKU:
CAB5676
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1251-1351 of human ATP7B (NP_001230111.1).
This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein is a monomer, and functions as a copper-transporting ATPase which exports copper out of the cells, such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants, encoding different isoforms with distinct cellular localizations, have been characterized. Mutations in this gene have been associated with Wilson disease which is characterized by copper accumulation.
Purification Method:
Affinity purification
Gene ID:
540
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat liver using ATP7B Rabbit pAb(CAB5676) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
