The ATP7A Polyclonal Antibody (CAB8399) is a valuable tool for researchers studying ATP7A, a copper-transporting ATPase protein essential for copper homeostasis. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By specifically targeting the ATP7A protein, this antibody allows for the detection and analysis of ATP7A expression in various cell types, making it an ideal choice for studies in molecular biology and metal metabolism research.ATP7A plays a critical role in copper transport within cells, impacting processes such as copper uptake, distribution, and excretion.
Dysregulation of ATP7A function can lead to copper-related disorders like Menkes disease and Wilson disease, highlighting the importance of studying this protein in both health and disease contexts. With its ability to accurately detect ATP7A expression, the ATP7A Polyclonal Antibody is a valuable asset for researchers seeking to understand the molecular mechanisms underlying copper metabolism and associated disorders.
Product Name:
ATP7A Rabbit Polyclonal Antibody
SKU:
CAB8399
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-79 of human ATP7A (NP_000043.4).
This gene encodes a transmembrane protein that functions in copper transport across membranes. This protein is localized to the trans Golgi network, where it is predicted to supply copper to copper-dependent enzymes in the secretory pathway. It relocalizes to the plasma membrane under conditions of elevated extracellular copper, and functions in the efflux of copper from cells. Mutations in this gene are associated with Menkes disease, X-linked distal spinal muscular atrophy, and occipital horn syndrome. Alternatively-spliced transcript variants have been observed.
Purification Method:
Affinity purification
Gene ID:
538
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat lung using ATP7A Rabbit pAb(CAB8399) at 1:1000 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime:60s.
