The ATP6V1G3 Polyclonal Antibody (CAB14443) is a valuable tool for researchers studying ATP6V1G3, a subunit of the vacuolar ATPase complex involved in various cellular processes such as vesicular trafficking, protein degradation, and pH homeostasis. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications.ATP6V1G3 is essential for maintaining cellular functions by regulating the acidification of lysosomes and endosomes, which are critical for processes like autophagy and protein degradation. Dysregulation of ATP6V1G3 has been linked to various diseases, including cancer and neurodegenerative disorders, making it a promising target for therapeutic interventions.
By utilizing the ATP6V1G3 Polyclonal Antibody, researchers can effectively detect and analyze ATP6V1G3 expression in different cell types, aiding in their investigations into the role of this subunit in cellular physiology and disease pathogenesis. This antibody is a valuable tool for studies in biochemistry, cell biology, and pharmacology, providing insights that may contribute to the development of novel treatment strategies for ATP6V1G3-related disorders.
Product Name:
ATP6V1G3 Rabbit Polyclonal Antibody
SKU:
CAB14443
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-118 of human ATP6V1G3 (NP_573569.1).
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'' and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes one of three G subunit proteins. Transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
127124
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from mouse kidney, using ATP6V1G3 Rabbit pAb (CAB14443) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
