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ATP6V1D Rabbit Polyclonal Antibody (CAB12940)

SKU:
CAB12940
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
$167 - $503
Frequently bought together:

Description

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ATP6V1D Rabbit Polyclonal Antibody (CAB12940)

The ATP6V1D Polyclonal Antibody (CAB12940) is a valuable tool for researchers studying ATP6V1D, a subunit of the V-ATPase enzyme complex involved in intracellular acidification and vesicular trafficking. This antibody, produced in rabbits, exhibits high reactivity with samples from various species and has been validated for use in Western blot and immunohistochemistry applications.ATP6V1D plays a crucial role in maintaining cellular pH balance and regulating membrane transport processes.

Dysregulation of ATP6V1D has been implicated in various diseases, including cancer, osteopetrosis, and renal tubular acidosis. By targeting ATP6V1D with this antibody, researchers can further explore its functions in cellular physiology and pathology, paving the way for potential therapeutic interventions targeting this important enzyme complex.

Product Name:ATP6V1D Rabbit Polyclonal Antibody
SKU:CAB12940
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-247 of human ATP6V1D (NP_057078.1).
Sequence:MSGK DRIE IFPS RMAQ TIMK ARLK GAQT GRNL LKKK SDAL TLRF RQIL KKII ETKM LMGE VMRE AAFS LAEA KFTA GDFS TTVI QNVN KAQV KIRA KKDN VAGV TLPV FEHY HEGT DSYE LTGL ARGG EQLA KLKR NYAK AVEL LVEL ASLQ TSFV TLDE AIKI TNRR VNAI EHVI IPRI ERTL AYII TELD ERER EEFY RLKK IQEK KKIL KEKS EKDL EQRR AAGE VLEP ANLL AEEK DEDL LFE
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:VATD; VMA8; ATP6M; ATP6V1D
Positive Sample:U-87MG,293T,HeLa,Mouse lung,Mouse brain
Conjugate:Unconjugated
Cellular Localization:Cytoplasmic side, Membrane, Peripheral membrane protein.
Calculated MW:28kDa
Observed MW:35kDa

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the V1 domain D subunit protein.

Purification Method:Affinity purification
Gene ID:51382
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using ATP6V1D Rabbit pAb (CAB12940) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

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