The ATP6V1C1 Polyclonal Antibody (CAB18253) is a valuable tool for researchers studying the ATP6V1C1 protein, a component of the vacuolar ATPase complex involved in cellular processes such as endocytosis, intracellular trafficking, and pH regulation. This antibody, derived from rabbit, is highly specific and reactive with human samples, making it suitable for use in Western blot applications.ATP6V1C1 is essential for maintaining cellular homeostasis and plays a crucial role in various physiological processes, including bone resorption, neurotransmission, and the regulation of cancer cell proliferation.
By targeting the ATP6V1C1 protein, researchers can gain insights into its function and potential therapeutic applications in diseases related to abnormal cellular acidification and ion regulation.The ATP6V1C1 Polyclonal Antibody is a reliable tool for investigating the intricacies of ATP6V1C1 biology in various cell types and tissues, making it a versatile asset for studies in cell biology, molecular biology, and cancer research. Its high specificity and sensitivity ensure accurate and reproducible results, allowing researchers to deepen their understanding of ATP6V1C1's role in health and disease.
Product Name:
ATP6V1C1 Rabbit Polyclonal Antibody
SKU:
CAB18253
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300-382 of human ATP6V1C1 (NP_001686.1).
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene is one of two genes that encode the V1 domain C subunit proteins and is found ubiquitously. This C subunit is analogous but not homologous to gamma subunit of F-ATPases. Previously, this gene was designated ATP6D.
Purification Method:
Affinity purification
Gene ID:
528
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ATP6V1C1 Rabbit pAb (CAB18253) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.
