The ATP6V1B1 Polyclonal Antibody (CAB6876) is a valuable tool for researchers studying ATP6V1B1, a subunit of the vacuolar ATPase enzyme involved in proton transport across membranes. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the ATP6V1B1 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an ideal choice for studies in molecular biology, cell biology, and biochemistry.
ATP6V1B1 is essential for maintaining cellular pH and ion balance, playing a crucial role in processes such as endocytosis, protein degradation, and neurotransmission. Dysregulation of ATP6V1B1 has been linked to various diseases including osteopetrosis, renal tubular acidosis, and certain cancers. By understanding the function and regulation of ATP6V1B1, researchers can gain insights into the pathophysiology of these conditions and potentially develop targeted therapies in the future.
Product Name:
ATP6V1B1 Rabbit Polyclonal Antibody
SKU:
CAB6876
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ATP6V1B1 (NP_001683.2).
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain B subunit isoforms and is found in the kidney. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness.
Purification Method:
Affinity purification
Gene ID:
525
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ATP6V1B1 antibody (CAB6876) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
