The ATP6V1A Polyclonal Antibody (CAB14707) is specifically designed for research involving the ATP6V1A protein, a crucial component of the vacuolar ATPase complex responsible for regulating intracellular pH and maintaining lysosomal function. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.By binding to the ATP6V1A protein, this antibody enables the detection and analysis of ATP6V1A in various cell types, making it an essential tool for studies in cell biology and molecular biology. The ATP6V1A protein is involved in multiple cellular processes, including endocytosis, protein degradation, and autophagy, highlighting its importance in maintaining cellular homeostasis.
Research into the function and regulation of the ATP6V1A protein is crucial for understanding its role in various physiological and pathological processes, such as lysosomal storage disorders, cancer progression, and neurodegenerative diseases. By using the ATP6V1A Polyclonal Antibody, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify novel therapeutic targets for intervention.
Product Name:
ATP6V1A Rabbit Polyclonal Antibody
SKU:
CAB14707
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 500-600 of human ATP6V1A (NP_001681.2).
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist.
Purification Method:
Affinity purification
Gene ID:
523
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from mouse brain, using ATP6V1A Rabbit pAb (CAB14707) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.
