The ATP6V1A Polyclonal Antibody (CAB14706) is a valuable tool for researchers studying ATP6V1A, a subunit of the V-type ATPase complex essential for maintaining cellular pH balance and membrane trafficking. This polyclonal antibody, produced in rabbits, exhibits high specificity and sensitivity for human samples, making it ideal for Western blot applications. By binding to the ATP6V1A protein, researchers can easily detect and analyze its expression in a variety of cell types.ATP6V1A is a key player in cellular processes such as endocytosis, vesicular trafficking, and lysosomal acidification, making it a crucial target for investigation in fields such as cell biology and cancer research.
Dysregulation of ATP6V1A has been linked to various diseases, including neurological disorders and cancer progression, highlighting its importance in cellular homeostasis and disease pathology. By utilizing the ATP6V1A Polyclonal Antibody, researchers can gain valuable insights into the function and regulation of this protein, paving the way for potential therapeutic interventions in related conditions.
Product Name:
ATP6V1A Rabbit Polyclonal Antibody
SKU:
CAB14706
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 500-600 of human ATP6V1A (NP_001681.2).
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist.
Purification Method:
Affinity purification
Gene ID:
523
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ATP6V1A Rabbit pAb (CAB14706) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.
