The ATP6V0D2 Polyclonal Antibody (PAC06257) is a valuable tool for researchers studying ATP6V0D2, a subunit of the V-ATPase complex involved in cellular processes such as endocytosis, lysosomal degradation, and pH regulation. This antibody, produced in rabbits, exhibits high specificity for human samples and is suitable for Western blot applications. By binding to ATP6V0D2, researchers can analyze and detect the protein in various cell types, making it an essential component for investigations in cell biology, cancer research, and drug discovery.
ATP6V0D2 is a crucial component of the V-ATPase complex, which plays a key role in maintaining cellular homeostasis by regulating intracellular pH, membrane trafficking, and protein degradation. Dysregulation of ATP6V0D2 has been linked to various diseases, including cancer, neurodegenerative disorders, and osteoporosis. Investigating the function of ATP6V0D2 provides valuable insights into these pathological conditions and may lead to the development of novel therapeutic strategies targeting the V-ATPase complex.
Western Blot. Positive WB detected in: A549 whole cell lysate, PC3 whole cell lysate. All lanes: ATP6V0D2 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 41 kDa. Observed band size: 41 kDa.
Immunofluorescence staining of HepG2 cells with PACO62527 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO62527 diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acid, fying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis (By similarity).
Synonyms:
V-type proton ATPase subunit d 2 (V-ATPase subunit d 2) (Vacuolar proton pump subunit d 2), ATP6V0D2
