The ATP6 Monoclonal Antibody is a highly specific research tool designed for studying the ATP6 protein, a key component of the V-type ATPase complex involved in cellular energy production. This monoclonal antibody, generated using hybridoma technology, demonstrates exceptional reactivity to human ATP6 and is validated for use in various applications, including Western blotting and immunofluorescence.ATP6, also known as the subunit a of V-ATPase, plays a crucial role in maintaining cellular pH balance and promoting lysosomal acidification. Dysregulation of ATP6 function has been linked to various pathological conditions, including neurodegenerative diseases and cancer.
By targeting ATP6 with this monoclonal antibody, researchers can investigate its expression and localization in different cell types, shedding light on its physiological functions and potential implications in disease development.Overall, the ATP6 Monoclonal Antibody offers a reliable tool for exploring the roles of ATP6 in cellular metabolism, organelle function, and disease pathogenesis. Its specificity and sensitivity make it a valuable asset for researchers in biochemistry, cell biology, and molecular medicine seeking to unravel the complexities of ATP6 biology.
Predicted to contribute to proton-transporting ATP synthase activity, rotational mechanism. Predicted to be involved in mitochondrial ATP synthesis coupled proton transport. Located in mitochondrion. Is expressed in several structures, including alimentary system; brain; heart; integumental system; and sensory organ. Human ortholog(s) of this gene implicated in Leber hereditary optic neuropathy; NARP syndrome; Parkinson's disease; multiple sclerosis; and systemic lupus erythematosus. Orthologous to human MT-ATP6 (mitochondrially encoded ATP synthase membrane subunit 6).
Purification Method:
Affinity purification
Gene ID:
17705
Clone Number:
ARC59849
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using ATP6 antibody (CAB23150) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
