The ATP5O Polyclonal Antibody (CAB8103) is a key tool for researchers studying ATP5O, a crucial subunit of mitochondrial ATP synthase. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the ATP5O protein, this antibody enables accurate detection and analysis in various cell types, making it an essential component for studies in the fields of biochemistry, cell biology, and mitochondrial function.
ATP5O is an essential component of the ATP synthase enzyme complex, which plays a vital role in cellular energy production through oxidative phosphorylation. Dysregulation of ATP5O has been linked to various diseases, including metabolic disorders and neurodegenerative conditions. By investigating the function and regulation of ATP5O using this antibody, researchers can gain valuable insights into the mechanisms of energy production in cells and potentially uncover new therapeutic targets for related diseases.
Product Name:
ATP5O Rabbit Polyclonal Antibody
SKU:
CAB8103
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 24-213 of human ATP5O (NP_001688.1).
The protein encoded by this gene is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. The encoded protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance.
Purification Method:
Affinity purification
Gene ID:
539
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using ATP5O Rabbit pAb (CAB8103) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
