The ATP5L2 Polyclonal Antibody (PAC023390) is a valuable tool for research involving ATP5L2, a protein involved in mitochondrial function and energy production. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for Western blot applications. By specifically binding to the ATP5L2 protein, researchers can accurately detect and analyze its expression in various cell types, making it an essential reagent for studies in cell biology and metabolism.ATP5L2, a subunit of the ATP synthase complex in mitochondria, plays a crucial role in ATP synthesis and cellular energy metabolism.
Dysregulation of ATP5L2 has been linked to various metabolic disorders, neurodegenerative diseases, and cancer. Understanding the function and regulation of ATP5L2 is vital for gaining insights into these pathological conditions and developing potential therapeutic strategies targeting mitochondrial dysfunction.Overall, the ATP5L2 Polyclonal Antibody serves as a reliable tool for investigating the role of ATP5L2 in cellular energy metabolism and its implications in various disease conditions, paving the way for advancements in mitochondrial biology research and potential therapeutic interventions.
Synthesized peptide derived from internal of human ATP5L2.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from A549 cells, using ATP5L2 antibody.
Immunofluorescence analysis of A549 cells, using ATP5L2 antibody.
Background:
Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain. Minor subunit located with subunit a in the membrane By similarity.
Synonyms:
ATP synthase subunit g 2; mitochondrial; ATPase subunit g 2; ATP5K2;
UniProt Protein Function:
ATP5L2: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane. Belongs to the ATPase g subunit family.Chromosomal Location of Human Ortholog: 22q13.2Cellular Component: mitochondrion
