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ATP5J Rabbit Polyclonal Antibody (CAB3751)

SKU:
CAB3751
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction
  • Signal Transduction Antibodies 2 Anti-ATP5J Antibody CAB3751
  • Signal Transduction Antibodies 2 Anti-ATP5J Antibody CAB3751
  • Signal Transduction Antibodies 2 Anti-ATP5J Antibody CAB3751
€139 - €419
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Description

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ATP5J Rabbit Polyclonal Antibody (CAB3751)

The ATP5J Polyclonal Antibody (CAB3751) is specifically designed for research involving ATP5J, a vital subunit of the ATP synthase complex responsible for cellular energy production. This antibody, produced from rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding to the ATP5J protein, researchers can effectively detect and analyze its expression in various cell types, making it an essential tool for studies in cell biology and metabolism.ATP5J plays a crucial role in the process of oxidative phosphorylation, where ATP is synthesized to provide energy for cellular functions.

Understanding the function and regulation of ATP5J is essential for unraveling the mechanisms underlying cellular metabolism, as dysregulation of ATP production has been linked to various diseases, including metabolic disorders and neurodegenerative conditions. By investigating ATP5J, researchers can gain valuable insights into energy metabolism and its implications for health and disease.

Product Name:ATP5J Rabbit Polyclonal Antibody
SKU:CAB3751
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-108 of human ATP5J (NP_001676.2).
Sequence:MILQ RLFR FSSV IRSA VSVH LRRN IGVT AVAF NKEL DPIQ KLFV DKIR EYKS KRQT SGGP VDAS SEYQ QELE RELF KLKQ MFGN ADMN TFPT FKFE DPKF EVIE KPQA
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:200 - 1:2000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200
Synonyms:F6; CF6; ATP5; ATPM; ATP5A; ATP5J
Positive Sample:SW480,THP-1,NCI-H460,22Rv1,Mouse kidney,Mouse heart
Conjugate:Unconjugated
Cellular Localization:Mitochondrion, Mitochondrion inner membrane.
Calculated MW:13kDa
Observed MW:13kDa

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo complex has nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the Fo complex. The F6 subunit is required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. This gene has 1 or more pseudogenes.

Purification Method:Affinity purification
Gene ID:522
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using ATP5J Rabbit pAb (CAB3751) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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