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ATP5I Rabbit Polyclonal Antibody (CAB16769)

SKU:
CAB16769
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
€139 - €419
Frequently bought together:

Description

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ATP5I Rabbit Polyclonal Antibody (CAB16769)

The ATP5I Rabbit Polyclonal Antibody (CAB16769) is a vital tool for researchers studying ATP5I, a subunit of the ATP synthase complex found in mitochondria. This antibody is specifically designed for use in Western blot applications and is highly reactive with human samples. By binding to the ATP5I protein, this antibody enables the detection and analysis of ATP synthase activity in various cell types.ATP5I plays a crucial role in cellular energy production through the generation of ATP, the primary energy currency of the cell. Dysregulation of ATP5I has been implicated in various diseases, including metabolic disorders and neurodegenerative diseases.

By studying the function and regulation of ATP5I, researchers can gain valuable insights into cellular metabolism and its implications in disease pathology.The ATP5I Rabbit Polyclonal Antibody is a valuable tool for researchers looking to delve into the intricate mechanisms of ATP synthesis and the role of ATP5I in cellular energy production. Its high reactivity and specificity make it an essential component in studies focusing on mitochondrial function, metabolism, and disease pathology. By understanding the intricacies of ATP5I, researchers can potentially uncover new therapeutic targets for a range of diseases.

Product Name:ATP5I Rabbit Polyclonal Antibody
SKU:CAB16769
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-69 of human ATP5I (NP_009031.1).
Sequence:MVPP VQVS PLIK LGRY SALF LGVA YGAT RYNY LKPR AEEE RRIA AEEK KKQD ELKR IARE LAED DSIL K
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200
Synonyms:ATP5I; ATP5K
Positive Sample:U-87MG,LO2,293T,HeLa
Cellular Localization:Mitochondrion, Mitochondrion inner membrane.
Calculated MW:8kDa
Observed MW:Refer to figures

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the e subunit of the Fo complex. Alternative splicing results in multiple transcript variants.

Purification Method:Affinity purification
Gene ID:521
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using ATP5I Rabbit pAb (CAB16769) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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