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ATP5F1 Antibody (CAB7645)

SKU:
CAB7645
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction
  • Signal Transduction Antibodies 3 Anti-ATP5F1 Antibody CAB7645
  • Signal Transduction Antibodies 3 Anti-ATP5F1 Antibody CAB7645
€139
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Description

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ATP5F1 Rabbit Polyclonal Antibody (CAB7645)

The ATP5F1 Polyclonal Antibody (CAB7645) is a valuable tool for research involving the ATP5F1 protein, a crucial component of the mitochondrial ATP synthase complex. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to ATP5F1, researchers can accurately detect and analyze the protein in various cell types, making it ideal for studies in biochemistry and cell biology.ATP5F1 is a key player in cellular energy production, as it helps to convert ADP into ATP in the mitochondria. Dysfunction of ATP5F1 has been linked to various metabolic disorders and diseases, making it an important target for research in areas such as mitochondrial dysfunction, neurodegenerative diseases, and metabolic syndromes.

Understanding the role of ATP5F1 is essential for developing targeted therapies that aim to restore proper cellular energy metabolism and function.Overall, the ATP5F1 Polyclonal Antibody is a valuable tool for researchers studying the intricate mechanisms of ATP synthesis and mitochondrial function, with potential applications in a wide range of biological and medical research fields.

Product Name:ATP5F1 Rabbit Polyclonal Antibody
SKU:CAB7645
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human ATP5F1 (NP_001679.2).
Sequence:MLSR VVLS AAAT AAPS LKNA AFLG PGVL QATR TFHT GQPH LVPV PPLP EYGG KVRY GLIP EEFF QFLY PKTG VTGP YVLG TGLI LYAL SKEI YVIS AETF TALS VLGV MVYG IKKY GPFV ADFA DKLN EQKL AQLE EAKQ ASIQ HIQN AIDT EKSQ QALV QKRH YLFD VQRN NIAM ALEV TYRE RLYR VYKE VKNR LDYH ISVQ NMMR RKEQ EHMI NWVE KHVV QSIS TQQE KETI AKCI ADLK L
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200
Synonyms:PIG47; ATP5F1
Positive Sample:THP-1,22Rv1,HepG2,Mouse liver,Mouse testis,Mouse spleen
Conjugate:Unconjugated
Cellular Localization:Mitochondrion, Mitochondrion inner membrane.
Calculated MW:29kDa
Observed MW:26kDa

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the b subunit of the proton channel.

Purification Method:Affinity purification
Gene ID:515
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using ATP5F1 antibody (CAB7645) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 60s.

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